Vitamin B12 CAS NO.68-19-9

Item	Standard	Result
Characteristics	Red crystal or crystalline hygroscopic powder, with no smell and no taste; Hygroscopicity.	Complies
Identification A	A361nm/A278nm:1.70-1.90	1.83
Identification A	A361nm/A550nm:3.15-3.40	3.25
Identification B	The retention time of major peak of the sample solution corresponds to that of the standard solution.	Complies
Infrared spectrum	The infrared spectrum of the sample should be consistent with the reference map.	Complies
Identification C	Positive	Complies
Loss on drying	≤12.0%	2.2%
Assay on dried basis	96.0%-102.0%	99.0%
Total impurities	≤3.0%	1.24%
7β,8β-lactocyanocobalamin	≤1.0%	0.24%
50-carboxycyanocobalamin	≤0.5%	0.08%
34-methylcyanocobalamin	≤2.0%	Not detected
32-carboxycyanocobalamin	≤1.0%	0.31%
8-Epi-cyanocobalamin	≤1.0%	0.22%
Other unidentified impurity	≤0.5	0.13%
Total aerobic bacteria count	≤1000cfu/g	20cfu/g
Mould & yeasts	≤100cfu/g	Not detected
E.Coli	Negative	Not detected
Endotoxin	≤0.4EU/mg	Complies
Residual solvents	Acetone≤0.5%	0.072%