IPTG (Isopropyl β-D-1-Thiogalactopyranoside) CAS NO.367-93-1

Isopropyl β-D-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.

IPTG, unlike allolactose, is not hydrolyzable by β-galactosidase, its concentration therefore remains constant in an experiment. For induction, a sterile, filtered 1 M solution of IPTG is typically added by 1:1000 dilution into a logarithmically growing bacterial culture, however, different final concentration of IPTG may also be used.

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Commonly used inducer for beta-Galactosidase activity in many bacterial gene sequences controlled by the lac operon. Based on this feature, when a pUC series vector DNA (or other vector with a lacZ gene DNA) deletion in lacZ host cells are transformed with the vector during DNA M13 phage were transfected or, if added to the medium in the plate X -gal and IPTG, since β- galactosidase complementarity of α-, may appear white depending on whether colonies (or plaques) and easily pick out recombinants. In addition, it can be used as having a lac or tac promoter expression vectors and other expression inducer. Now more and more areas have been used IPTG, undergoing tremendous role in the field of production, pharmaceutical, biotechnology, etc. for companies to bring greater efficiency, improve product quality and shorten the reaction time.